pdlim7 primary antibody  (FineTest Biotech Inc)

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: PDLIM7 expression is increased in renal clear cell carcinoma tissue samples and cells and correlates with poor prognosis. ( A – C ) The public database shows elevated expression of PDLIM7 in ccRCC. ( D ) Immunohistochemical staining shows elevated expression of PDLIM7 in ccRCC. ( E ) PDLIM7 expression in ccRCC cells was significantly higher than that in normal cells HK2. Scale bar:10 µm and 5 µm ( F , G ) Effect of PDLIM7 on OS and PFS in ccRCC patients.

Article Snippet: PDLIM7 primary antibody(1:100,finetest,China), a DAB-enhanced chromogenic solution, and a rabbit two-step assay kit were obtained from Beijing Zhongsui Jinqiao Company.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: PDLIM7 promotes cell proliferation, migration, and invasion in ccRCC. ( A ) Validation of western blot assay for transfection with sh-PDLIM7 in 769-p and Caki-1 cells. ( B ) Cloning assay for ccRCC cell viability. ( C ) Detection of cellular wound healing capacity by scratch assay. Scale bar:500 µm ( D ) Transwell assay for cell migration and invasion. Scale bar:10 µm ( E ) Western blot for EMT-related protein expression.

Article Snippet: PDLIM7 primary antibody(1:100,finetest,China), a DAB-enhanced chromogenic solution, and a rabbit two-step assay kit were obtained from Beijing Zhongsui Jinqiao Company.

Techniques: Migration, Biomarker Discovery, Western Blot, Transfection, Cloning, Wound Healing Assay, Transwell Assay, Expressing

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: KEGG/GO functional enrichment and drug sensitivity. ( A ) Functional enrichment of PDLIM7 and related genes KEGG/GO. ( B ) PDLIM7 and drug correlation.

Article Snippet: PDLIM7 primary antibody(1:100,finetest,China), a DAB-enhanced chromogenic solution, and a rabbit two-step assay kit were obtained from Beijing Zhongsui Jinqiao Company.

Techniques: Functional Assay

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: PDLIM7 expression is increased in renal clear cell carcinoma tissue samples and cells and correlates with poor prognosis. ( A – C ) The public database shows elevated expression of PDLIM7 in ccRCC. ( D ) Immunohistochemical staining shows elevated expression of PDLIM7 in ccRCC. ( E ) PDLIM7 expression in ccRCC cells was significantly higher than that in normal cells HK2. Scale bar:10 µm and 5 µm ( F , G ) Effect of PDLIM7 on OS and PFS in ccRCC patients.

Article Snippet: The membrane was blocked with 6% skimmed milk for 2 h, followed by the addition of primary rabbit anti-human antibodies: PDLIM7 (1:1000, finetest, China), E-cadherin (1:2000, immunoway, China), N-cadherin (1:2000, immunoway, China), and GAPDH (1:5000, immunoway, China).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: PDLIM7 promotes cell proliferation, migration, and invasion in ccRCC. ( A ) Validation of western blot assay for transfection with sh-PDLIM7 in 769-p and Caki-1 cells. ( B ) Cloning assay for ccRCC cell viability. ( C ) Detection of cellular wound healing capacity by scratch assay. Scale bar:500 µm ( D ) Transwell assay for cell migration and invasion. Scale bar:10 µm ( E ) Western blot for EMT-related protein expression.

Article Snippet: The membrane was blocked with 6% skimmed milk for 2 h, followed by the addition of primary rabbit anti-human antibodies: PDLIM7 (1:1000, finetest, China), E-cadherin (1:2000, immunoway, China), N-cadherin (1:2000, immunoway, China), and GAPDH (1:5000, immunoway, China).

Techniques: Migration, Biomarker Discovery, Western Blot, Transfection, Cloning, Wound Healing Assay, Transwell Assay, Expressing

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: KEGG/GO functional enrichment and drug sensitivity. ( A ) Functional enrichment of PDLIM7 and related genes KEGG/GO. ( B ) PDLIM7 and drug correlation.

Article Snippet: The membrane was blocked with 6% skimmed milk for 2 h, followed by the addition of primary rabbit anti-human antibodies: PDLIM7 (1:1000, finetest, China), E-cadherin (1:2000, immunoway, China), N-cadherin (1:2000, immunoway, China), and GAPDH (1:5000, immunoway, China).

Techniques: Functional Assay